Bioactive nano-selenium antagonizes cobalt nanoparticle-mediated oxidative stress via the Keap1-Nrf2-ARE signaling pathway

Abstract At present, no effective treatment exists for the clinical toxicity of cobalt nanoparticles (CoNPs, 30 nm) after metal-on-metal (MOM) artificial joint replacement. As such, a better understanding CoNPs-toxicity mechanism is necessary and urgent development safe detoxification drugs. Our purpose was to explore role bioactive nano-selenium (BNS, > 97%) in antagonizing CoNPs its through Keap1-Nrf2-ARE signaling pathway. To examine BNS detoxification, we exposed HUVEC cells 24 h, before measuring cell activity, reactive oxygen species (ROS), GSH level, inflammatory factors, KNA pathway-related transcript protein expression. stimulate intracellular inflammation ROS production bring about significant downregulation cellular activity level. Conversely, reduces generation suppresses factors within reduce CoNPs-mediated cytotoxicity, possibly via Based on our results, antagonizes toxic effects by suppressing research provides new insight into explores potential therapy. Trial registration: human participant.